Open AccessCrystallization and preliminary crystallographic studies of CbsA, a secretory exoglucanase from Xanthomonas oryzae pv. oryzae
Author(s) -
Kumar Sushil,
Haque Asfarul S.,
Jha Gopaljee,
Sonti Ramesh V.,
Sankaranarayanan Rajan
Publication year - 2012
Publication title -
acta crystallographica section f
Language(s) - English
Resource type - Journals
ISSN - 1744-3091
DOI - 10.1107/s1744309112034197
Subject(s) - xanthomonas oryzae , crystallization , xanthomonas oryzae pv. oryzae , crystallography , chemistry , biology , microbiology and biotechnology , materials science , organic chemistry , pathogen
The bacterial pathogen Xanthomonas oryzae pv. oryzae causes bacterial leaf blight, a serious disease of rice. The secreted exoglucanase CbsA is an important virulence factor of this pathogen. It belongs to the glycosyl hydrolase 6 family of proteins based on the carbohydrate‐active enzyme (CAZY) classification. In this study, CbsA has been overexpressed, purified and crystallized. The crystal diffracted to a resolution of 1.86 Å and belonged to space group P 2 1 2 1 2 1 . It contained one monomer per asymmetric unit, with a solvent content of 45.8%.