Open AccessCrystallization and preliminary diffraction studies of SFC‐1, a carbapenemase conferring antibiotic resistance
Author(s) -
Hong MyoungKi,
Lee Jae Jin,
Wu Xing,
Kim JinKwang,
Jeong Byeong Chul,
Pham TanViet,
Kim SeungHwan,
Lee Sang Hee,
Kang LinWoo
Publication year - 2012
Publication title -
acta crystallographica section f
Language(s) - English
Resource type - Journals
ISSN - 1744-3091
DOI - 10.1107/s1744309112033702
Subject(s) - aztreonam , orthorhombic crystal system , antibiotics , cephalosporin , microbiology and biotechnology , antibiotic resistance , lactam , crystallization , antimicrobial , chemistry , stereochemistry , biology , crystallography , crystal structure , organic chemistry , imipenem
SFC‐1, a class A carbapenemase that confers antibiotic resistance, hydrolyzes the β‐lactam rings of β‐lactam antibiotics (carbapenems, cephalosporins, penicillins and aztreonam). SFC‐1 presents an enormous challenge to infection control, particularly in the eradication of Gram‐negative pathogens. As SFC‐1 exhibits a remarkably broad substrate range, including β‐lactams of all classes, the enzyme is a potential target for the development of antimicrobial agents against pathogens producing carbapenemases. In this study, SFC‐1 was cloned, overexpressed, purified and crystallized. The SFC‐1 crystal diffracted to 1.6 Å resolution and belonged to the orthorhombic space group P 2 1 2 1 2 1 , with unit‐cell parameters a = 65.8, b = 68.3, c = 88.8 Å. Two molecules are present in the asymmetric unit, with a corresponding V M of 1.99 Å 3 Da −1 and a solvent content of 38.1%.