Open AccessCrystallization and preliminary X‐ray analysis of Chandipura virus glycoprotein G
Author(s) -
Baquero Eduard,
Buonocore Linda,
Rose John K.,
Bressanelli Stéphane,
Gaudin Yves,
Albertini Aurélie A.
Publication year - 2012
Publication title -
acta crystallographica section f
Language(s) - English
Resource type - Journals
ISSN - 1744-3091
DOI - 10.1107/s1744309112030151
Subject(s) - ectodomain , lipid bilayer fusion , glycoprotein , trimer , lassa virus , vesicular stomatitis virus , herpesvirus glycoprotein b , fusion protein , crystallography , measles virus , biology , chemistry , virus , biochemistry , biophysics , recombinant dna , virology , viral entry , viral replication , dimer , receptor , organic chemistry , gene , measles , vaccination
Fusion in members of the Rhabdoviridae virus family is mediated by the G glycoprotein. At low pH, the G glycoprotein catalyzes fusion between viral and endosomal membranes by undergoing a major conformational change from a pre‐fusion trimer to a post‐fusion trimer. The structure of the G glycoprotein from vesicular stomatitis virus (VSV G), the prototype of Vesiculovirus , has recently been solved in its trimeric pre‐fusion and post‐fusion conformations; however, little is known about the structural details of the transition. In this work, a soluble form of the ectodomain of Chandipura virus G glycoprotein (CHAV G th ) was purified using limited proteolysis of purified virus; this soluble ectodomain was also crystallized. This protein shares 41% amino‐acid identity with VSV G and thus its structure could provide further clues about the structural transition of rhabdoviral glycoproteins induced by low pH. Crystals of CHAV G th obtained at pH 7.5 diffracted X‐rays to 3.1 Å resolution. These crystals belonged to the orthorhombic space group P 2 1 2 1 2, with unit‐cell parameters a = 150.3, b = 228.2, c = 78.8 Å. Preliminary analysis of the data based on the space group and the self‐rotation function indicated that there was no trimeric association of the protomers. This unusual oligomeric status could result from the presence of fusion intermediates in the crystal.