Open AccessPurification, crystallization and preliminary crystallographic analysis of banyan peroxidase
Author(s) -
Sharma Anurag,
Palm Gottfried J.,
Kumari Moni,
Panjikar Santosh,
Jagannadham M. V.,
Hinrichs Winfried
Publication year - 2012
Publication title -
acta crystallographica section f
Language(s) - English
Resource type - Journals
ISSN - 1744-3091
DOI - 10.1107/s1744309112026097
Subject(s) - peroxidase , crystallization , crystallography , trigonal crystal system , thermal stability , diffraction , materials science , chemistry , crystal structure , enzyme , organic chemistry , physics , optics
Plant peroxidases are extensively used in a wide range of biotechnological applications owing to their high environmental and thermal stability. A new peroxidase, named banyan peroxidase, was purified from the latex of Ficus benghalensis and crystallized. X‐ray diffraction data were collected from native crystals and from bromide and xenon derivatives to resolutions of up to 1.66 Å in the trigonal space group P 3 2 21, with unit‐cell parameters a = b = 73.1, c = 164.6 Å. The anomalous signal of the intrinsic iron and calcium ions was sufficient for structure solution by SAD, although the sequence is not yet known.