Open AccessCloning, expression, purification and preliminary X‐ray analysis of a putative metagenome‐derived lipase
Author(s) -
Fersini Francesco,
Dall'Antonia Yuliya,
Chow Jennifer,
Streit Wolfgang R.,
MuellerDieckmann Jochen
Publication year - 2012
Publication title -
acta crystallographica section f
Language(s) - English
Resource type - Journals
ISSN - 1744-3091
DOI - 10.1107/s1744309112025651
Subject(s) - lipase , cloning (programming) , esterase , metagenomics , molecular cloning , biology , recombinant dna , resolution (logic) , computational biology , gene , genomic library , microbiology and biotechnology , genetics , biochemistry , enzyme , peptide sequence , computer science , programming language , artificial intelligence
LipS is a novel thermostable putative lipase that was isolated from a metagenomic library using functional screening methods. The corresponding gene shows high similarity to that encoding a putative but uncharacterized esterase from Symbiobacterium thermophilum IAM14863 (99% nucleotide‐sequence similarity). Two different constructs of the recombinant lipase were crystallized. Crystals belonging to space group P 4 2 2 1 2 diffracted X‐ray radiation to 2.8 Å resolution and crystals belonging to space group P 4 diffracted to 2.0 Å resolution. The most probable content of their asymmetric units were two molecules ( P 4 2 2 1 2) and four or five molecules ( P 4), respectively.