Open AccessCrystallization and preliminary crystallographic analysis of the C‐terminal domain of MamM, a magnetosome‐associated protein from Magnetospirillum gryphiswaldense MSR‐1
Author(s) -
Zeytuni Natalie,
Offer Tal,
Davidov Geula,
Zarivach Raz
Publication year - 2012
Publication title -
acta crystallographica section f
Language(s) - English
Resource type - Journals
ISSN - 1744-3091
DOI - 10.1107/s1744309112025638
Subject(s) - magnetosome , magnetotactic bacteria , crystallography , crystallization , biomineralization , chemistry , biology , bacteria , genetics , paleontology , organic chemistry
MamM is a unique magnetosome‐associated protein that shares substantial homology with cation diffusion facilitator (CDF) proteins, a group of heavy‐metal‐ion efflux transporters that participate in metal‐ion homeostasis in all domains of life. Magnetotactic bacteria utilize CDF proteins in iron‐oxide biomineralization and in magnetosome formation. Here, the crystallization and preliminary X‐ray analysis of recombinant Magnetospirillum gryphiswaldense MamM is reported. The C‐terminal domain of MamM was crystallized in the orthorhombic space group C 222 1 , with unit‐cell parameters a = 37.1, b = 94.0, c = 53.3 Å. X‐ray diffraction data were collected to a resolution of 2.0 Å.