Structure determination by multiple‐wavelength anomalous dispersion (MAD) at the Pr  L  III  edge | Zendy

Puehringer Sandra | Zendy; Hellmig Michael | Zendy; Liu Sunbin | Zendy; Weiss Manfred S. | Zendy; Wahl Markus C. | Zendy; Mueller Uwe | Zendy

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Structure determination by multiple‐wavelength anomalous dispersion (MAD) at the Pr L III edge

Author(s) -

Puehringer Sandra,

Hellmig Michael,

Liu Sunbin,

Weiss Manfred S.,

Wahl Markus C.,

Mueller Uwe

Publication year - 2012

Publication title -

acta crystallographica section f

Language(s) - English

Resource type - Journals

ISSN - 1744-3091

DOI - 10.1107/s1744309112025456

Subject(s) - wavelength , phaser , lanthanide , enhanced data rates for gsm evolution , crystallography , dispersion (optics) , anomalous scattering , physics , optics , molecular physics , chemistry , diffraction , computer science , ion , telecommunications , quantum mechanics

The use of longer X‐ray wavelengths in macromolecular crystallography has grown significantly over the past few years. The main reason for this increased use of longer wavelengths has been to utilize the anomalous signal from sulfur, providing a means for the experimental phasing of native proteins. Here, another possible application of longer X‐ray wavelengths is presented: MAD at the L III edges of various lanthanide compounds. A first experiment at the L III edge of Pr was conducted on HZB MX beamline BL14.2 and resulted in the successful structure determination of the C‐terminal domain of a spliceosomal protein. This experiment demonstrates that L III edges of lanthanides constitute potentially attractive targets for long‐wavelength MAD experiments.

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