Open AccessExpression, purification, crystallization and preliminary crystallographic analysis of the phosphoglycerate kinase from Acinetobacter baumannii
Author(s) -
Baretta Kayla,
Garen Craig,
Yin Jiang,
James Michael N. G.
Publication year - 2012
Publication title -
acta crystallographica section f
Language(s) - English
Resource type - Journals
ISSN - 1744-3091
DOI - 10.1107/s1744309112020222
Subject(s) - crystallization , acinetobacter baumannii , microbiology and biotechnology , crystallography , materials science , computational biology , chemistry , biology , bacteria , genetics , organic chemistry , pseudomonas aeruginosa
Acinetobacter baumannii is a common multidrug‐resistant clinical pathogen that is often found in hospitals. The A. baumannii phosphoglycerate kinase ( Ab PGK) is involved in the key energy‐producing pathway of glycolysis and presents a potential target for antibiotic development. Ab PGK has been expressed and purified; it was crystallized using lithium sulfate as the precipitant. The Ab PGK crystals belonged to space group P 222 1 . They diffracted to a resolution of 2.5 Å using synchrotron radiation at the Canadian Light Source.