Open AccessCrystallization of the novel S ‐adenosyl‐ l ‐methionine‐dependent C ‐methyltransferase CouO from Streptomyces rishiriensis and preliminary diffraction data analysis
Author(s) -
Lyskowski Andrzej,
Tengg Martin,
Steinkellner Georg,
Schwab Helmut,
GruberKhadjawi Mandana,
Gruber Karl
Publication year - 2012
Publication title -
acta crystallographica section f
Language(s) - English
Resource type - Journals
ISSN - 1744-3091
DOI - 10.1107/s1744309112017137
Subject(s) - crystallization , recombinant dna , methyltransferase , resolution (logic) , methionine , chemistry , crystallography , streptomyces , diffraction , stereochemistry , methylation , microbiology and biotechnology , biochemistry , biology , physics , bacteria , dna , organic chemistry , genetics , gene , optics , amino acid , artificial intelligence , computer science
Recombinant Q9F8T9 protein from Streptomyces rishiriensis (CouO), an S ‐adenosyl‐ l ‐methionine‐dependent C ‐methyltransferase, has been successfully cloned, expressed and purified. CouO was crystallized from a single condition in the Morpheus crystallization screen. A vitrified crystal diffracted to 2.05 Å resolution and belonged to space group P 2 1 , with unit‐cell parameters a = 33.02, b = 82.87, c = 76.77 Å, β = 96.93°.