Open AccessExpression, purification and crystallization of the Crimean–Congo haemorrhagic fever virus nucleocapsid protein
Author(s) -
Carter S. D.,
Barr J. N.,
Edwards T. A.
Publication year - 2012
Publication title -
acta crystallographica section f
Language(s) - English
Resource type - Journals
ISSN - 1744-3091
DOI - 10.1107/s1744309112009736
Subject(s) - virology , crystallization , recombinant dna , biology , circular dichroism , virus , crystallography , microbiology and biotechnology , chemistry , biochemistry , organic chemistry , gene
Crimean–Congo haemorrhagic fever virus (CCHFV) is a member of the Nairovirus genus within the Bunyaviridae family of segmented negative‐sense RNA viruses. This paper describes the expression, purification and crystallization of full‐length CCHFV nucleocapsid (N) protein and the collection of a 2.1 Å resolution X‐ray diffraction data set using synchrotron radiation. Crystals of the CCHFV N protein belonged to space group C 2, with unit‐cell parameters a = 150.38, b = 72.06, c = 101.23 Å, β = 110.70° and two molecules in the asymmetric unit. Circular‐dichroism analysis provided insight into the secondary structure, whilst gel‐filtration analysis revealed possible oligomeric states of the N protein. Structural determination is ongoing.