Open AccessInitial crystallographic studies of a small heat‐shock protein from Xylella fastidiosa
Author(s) -
Tada Susely F. S.,
Saraiva Antonio Marcos,
Lorite Gabriela S.,
RosselliMurai Luciana K.,
Pelloso Alexandre César,
dos Santos Marcelo Leite,
Trivella Daniela B. B.,
Cotta Mônica A.,
de Souza Anete Pereira,
Aparicio Ricardo
Publication year - 2012
Publication title -
acta crystallographica section f
Language(s) - English
Resource type - Journals
ISSN - 1744-3091
DOI - 10.1107/s1744309112009347
Subject(s) - xylella fastidiosa , recombinant dna , heat shock protein , crystallography , biology , escherichia coli , chemistry , microbiology and biotechnology , biochemistry , bacteria , genetics , gene
The ORF XF2234 in the Xylella fastidiosa genome was identified as encoding a small heat‐shock protein of 17.9 kDa (HSP17.9). HSP17.9 was found as one of the proteins that are induced during X. fastidiosa proliferation and infection in citrus culture. Recombinant HSP17.9 was crystallized and surface atomic force microscopy experiments were conducted with the aim of better characterizing the HSP17.9 crystals. X‐ray diffraction data were collected at 2.7 Å resolution. The crystal belonged to space group P 4 3 22, with unit‐cell parameters a = 68.90, b = 68.90, c = 72.51 Å, and is the first small heat‐shock protein to crystallize in this space group.