Open AccessStructure of the tetradecanucleotide d(CCCCGGTACCGGGG) 2 as an A‐DNA duplex
Author(s) -
Mandal Pradeep Kumar,
Venkadesh Sarkarai,
Gautham Namasivayam
Publication year - 2012
Publication title -
acta crystallographica section f
Language(s) - English
Resource type - Journals
ISSN - 1744-3091
DOI - 10.1107/s174430911200869x
Subject(s) - duplex (building) , crystallography , tetragonal crystal system , crystal structure , molecule , sequence (biology) , dna , base pair , resolution (logic) , materials science , chemistry , computer science , biochemistry , organic chemistry , artificial intelligence
The crystal structure of the tetradecanucleotide sequence d(CCCCGGTACCGGGG) 2 has been determined at 2.5 Å resolution in the tetragonal space group P 4 1 . This sequence was designed with the expectation of a four‐way junction. However, the sequence crystallized as an A‐DNA duplex and represents more than one full turn of the A‐helix. The crystallographic asymmetric unit consists of one tetradecanucleotide duplex. The structural parameters of the A‐type DNA duplex structure and the crystal‐packing arrangement are described. One Mn 2+ ion was identified with direct coordination to the N7 position of G 13 and a water molecule at the major‐groove side of the C 2 ·G 13 base pair.