Open AccessCocrystallization and preliminary crystallographic analysis of an inactive MaoC‐like hydratase mutant with the substrate crotonyl‐CoA
Author(s) -
Wang Huizheng,
Zhu Jie,
Song Weiwei,
Zhang Xiuguo
Publication year - 2012
Publication title -
acta crystallographica section f
Language(s) - English
Resource type - Journals
ISSN - 1744-3091
DOI - 10.1107/s1744309112007671
Subject(s) - mutant , substrate (aquarium) , crystallography , stereochemistry , chemistry , materials science , biochemistry , biology , gene , ecology
MaoC‐like hydratase (MaoC) is a recently identified enzyme involved in the biosynthetic pathway of polyhydroxyalkanoates (PHAs), which are completely biodegradable polymers used to produce green plastics. The inactive mutant D194N‐MaoC was crystallized in the presence of the substrate crotonyl‐CoA. Crystals were grown in a number of conditions, but only those produced using 20%( v / v ) ethylene glycol were suitable for structural studies. Data were collected to 2.10 Å resolution using X‐radiation and the crystal belonged to the orthorhombic space group P 2 1 2 1 2 1 , with unit‐cell parameters a = 81.40, b = 82.58, c = 123.99 Å.