Open AccessCrystallization and preliminary X‐ray analysis of the subclass B3 metallo‐β‐lactamase SMB‐1 that confers carbapenem resistance
Author(s) -
Wachino Junichi,
Yamaguchi Yoshihiro,
Mori Shigetarou,
Yamagata Yuriko,
Arakawa Yoshichika,
Shibayama Keigo
Publication year - 2012
Publication title -
acta crystallographica section f
Language(s) - English
Resource type - Journals
ISSN - 1744-3091
DOI - 10.1107/s1744309112004691
Subject(s) - subclass , crystallization , escherichia coli , crystal (programming language) , crystallography , molecule , chemistry , materials science , biology , biochemistry , genetics , gene , organic chemistry , computer science , antibody , programming language
The carbapenem‐hydrolyzing subclass B3 metallo‐β‐lactamase SMB‐1 was expressed in Escherichia coli and purified. Diffraction data were collected from two types of SMB‐1 crystals that were obtained under different conditions. One crystal (SMB‐1a) belonged to the trigonal space group P 3 1 with unit‐cell parameters a = b = 67.83, c = 48.67 Å, while the other crystal (SMB‐1b) also belonged to space group P 3 1 but with unit‐cell parameters a = b = 67.25, c = 46.83 Å. Both crystals contained one molecule per asymmetric unit. Initial phases were determined by molecular replacement; further refinement and model building are in progress.