Open AccessExpression, purification, crystallization and preliminary X‐ray diffraction analysis of a galactose 1‐phosphate uridylyltransferase from the hyperthermophilic archaeon Pyrobaculum aerophilum
Author(s) -
Satomura Takenori,
Hiraki Akihiro,
Kawai Tomoyuki,
Kawakami Ryushi,
Ohshima Toshihisa,
Sakuraba Haruhiko
Publication year - 2012
Publication title -
acta crystallographica section f
Language(s) - English
Resource type - Journals
ISSN - 1744-3091
DOI - 10.1107/s1744309112003880
Subject(s) - tetragonal crystal system , crystallization , polyethylene glycol , materials science , chemistry , crystallography , biochemistry , crystal structure , organic chemistry
A galactose 1‐phosphate uridylyltransferase from the hyperthermophilic archaeon Pyrobaculum aerophilum was crystallized using the sitting‐drop vapour‐diffusion method with polyethylene glycol 8000 as the precipitant. The crystals belonged to the tetragonal space group P 4 1 , with unit‐cell parameters a = b = 73.3, c = 126.1 Å, and diffracted to 2.73 Å resolution on beamline BL5A at the Photon Factory. The overall R merge was 7.3% and the data completeness was 99.8%.