Open AccessCrystallization and preliminary X‐ray analysis of alginate importer from Sphingomonas sp. A1
Author(s) -
Maruyama Yukie,
Itoh Takafumi,
Nishitani Yu,
Mikami Bunzo,
Hashimoto Wataru,
Murata Kousaku
Publication year - 2012
Publication title -
acta crystallographica section f
Language(s) - English
Resource type - Journals
ISSN - 1744-3091
DOI - 10.1107/s1744309112001893
Subject(s) - crystallization , x ray , materials science , sphingomonas , crystallography , chemistry , physics , biochemistry , optics , organic chemistry , gene , 16s ribosomal rna
Sphingomonas sp. A1 directly incorporates alginate polysaccharides through a `superchannel' comprising a pit on the cell surface, alginate‐binding proteins in the periplasm and an ABC transporter (alginate importer) in the inner membrane. Alginate importer, consisting of four subunits, AlgM1, AlgM2 and two molecules of AlgS, was crystallized in the presence of the binding protein AlgQ2. Preliminary X‐ray analysis showed that the crystal diffracted to 3.3 Å resolution and belonged to space group P 2 1 2 1 2 1 , with unit‐cell parameters a = 72.5, b = 136.8, c = 273.3 Å, suggesting the presence of one complex in the asymmetric unit.