Open AccessCrystallization and preliminary X‐ray crystallographic studies of the outer membrane cytochrome OmcA from Shewanella oneidensis MR‐1
Author(s) -
Tomanicek S. J.,
Johs A.,
Sawhney M. S.,
Shi L.,
Liang L.
Publication year - 2012
Publication title -
acta crystallographica section f
Language(s) - English
Resource type - Journals
ISSN - 1744-3091
DOI - 10.1107/s1744309111046082
Subject(s) - shewanella oneidensis , crystallization , crystallography , chemistry , electron transfer , cytochrome , electron microprobe , materials science , bacteria , biology , biochemistry , mineralogy , organic chemistry , genetics , enzyme
The outer membrane cytochrome OmcA functions as a terminal metal reductase in the dissimilatory metal‐reducing bacterium Shewanella oneidensis MR‐1. The ten‐heme centers shuttle electrons from the transmembrane donor complex to extracellular electron acceptors. Here, the crystallization and preliminary crystallographic analysis of OmcA are reported. Crystals of OmcA were grown by the sitting‐drop vapor‐diffusion method using PEG 20 000 as a precipitant. The OmcA crystals belonged to space group P 2 1 , with unit‐cell parameters a = 93.0, b = 246.0, c = 136.6 Å, α = 90, β = 97.8, γ = 90°. X‐ray diffraction data were collected to a maximum resolution of 3.25 Å.