Open AccessSalvage and storage of infectious disease protein targets in the SSGCID high‐throughput crystallization pathway using microfluidics
Author(s) -
Christensen Jeff,
Gerdts Cory J.,
Clifton Mathew C.,
Stewart Lance
Publication year - 2011
Publication title -
acta crystallographica section f
Language(s) - English
Resource type - Journals
ISSN - 1744-3091
DOI - 10.1107/s1744309111023232
Subject(s) - crystallization , protein crystallization , microfluidics , spark plug , materials science , drop (telecommunication) , throughput , diffraction , chemistry , chemical engineering , nanotechnology , computer science , operating system , organic chemistry , physics , optics , telecommunications , engineering , wireless , thermodynamics
The MPCS Plug Maker is a microcapillary‐based protein‐crystallization system for generating diffraction‐ready crystals from nanovolumes of protein. Crystallization screening using the Plug Maker was used as a salvage pathway for proteins that failed to crystallize during the initial observation period using the traditional sitting‐drop vapor‐diffusion method. Furthermore, the CrystalCards used to store the crystallization experiments set up by the Plug Maker are shown be a viable container for long‐term storage of protein crystals without a discernable loss of diffraction quality with time. Use of the Plug Maker with SSGCID proteins is demonstrated to be an effective crystal‐salvage and storage method.