Open AccessCrystallization and preliminary X‐ray studies of a galactose‐specific lectin from the seeds of Spatholobus parviflorus
Author(s) -
Geethanandan K.,
Abhilash Joseph,
Bharath S. R.,
Sadasivan C.,
Haridas M.
Publication year - 2011
Publication title -
acta crystallographica section f
Language(s) - English
Resource type - Journals
ISSN - 1744-3091
DOI - 10.1107/s174430911101387x
Subject(s) - lectin , crystallization , legume , galactose , chemistry , crystallography , botany , materials science , biology , biochemistry , organic chemistry
A galactose‐specific seed lectin was purified from the legume Spatholobus parviflorus and crystallized using the hanging‐drop vapour‐diffusion technique. The crystals belonged to space group P 1, with unit‐cell parameters a = 60.998, b = 60.792, c = 78.179 Å, α = 101.32, β = 91.38, γ = 104.32°. X‐ray diffraction data were collected under cryoconditions (100 K) to a resolution of 2.04 Å using a MAR image‐plate detector system mounted on a rotating‐anode X‐ray (Cu K α) generator. Molecular replacement using legume‐lectin coordinates as a search model gave a tetrameric structure.