Open AccessPurification, crystallization and preliminary X‐ray crystallographic analysis of the putative Vibrio parahaemolyticus resuscitation‐promoting factor YeaZ
Author(s) -
Aydin Inci,
Dimitropoulos Alexandra,
Chen Shih Hsun,
Thomas Connor,
Roujeinikova Anna
Publication year - 2011
Publication title -
acta crystallographica section f
Language(s) - English
Resource type - Journals
ISSN - 1744-3091
DOI - 10.1107/s1744309111010219
Subject(s) - vibrio parahaemolyticus , monoclinic crystal system , litopenaeus , viable but nonculturable , microbiology and biotechnology , crystallization , polyethylene glycol , crystallography , materials science , biology , chemistry , bacteria , crystal structure , biochemistry , organic chemistry , shrimp , genetics , fishery
Vibrio parahaemolyticus is a human pathogen associated with gastroenteritis caused by the ingestion of contaminated raw seafood. V. parahaemolyticus is able to survive exposure to low temperatures typical of those used for the refrigeration of foods by entering a viable but nonculturable (VBNC) state. The VBNC cells can regain culturability and renewed ability to cause infection upon temperature upshift. The resuscitation‐promoting factor protein (Rpf, YeaZ) plays a key role in reactivation of growth. Crystals of V. parahaemolyticus YeaZ have been grown using the hanging‐drop vapour‐diffusion method with polyethylene glycol as a precipitating agent. The crystals belonged to the primitive monoclinic space group P 2 1 , with unit‐cell parameters a = 81.7, b = 63.8, c = 82.3 Å, β = 105° and four subunits in the asymmetric unit. A complete X‐ray diffraction data set was collected from a single crystal to 3.1 Å resolution.