Open AccessPurification, crystallization and preliminary X‐ray crystallographic analysis of MIL, a glycosylated jacalin‐related lectin from mulberry ( Morus indica ) latex
Author(s) -
Patel Ashok K.,
Singh Vijay K.,
Bergmann Ulrich,
Jagannadham Medicherla V.,
Kursula Petri
Publication year - 2011
Publication title -
acta crystallographica section f
Language(s) - English
Resource type - Journals
ISSN - 1744-3091
DOI - 10.1107/s174430911101013x
Subject(s) - jacalin , lectin , crystallization , tetramer , crystallography , chemistry , resolution (logic) , x ray crystallography , protein crystallization , diffraction , biochemistry , organic chemistry , physics , enzyme , artificial intelligence , computer science , optics
A quantitatively major protein has been purified from the latex of Morus indica . The purified previously uncharacterized protein, M. indica lectin (MIL), was further shown to be a glycosylated tetramer and belongs to the family of jacalin‐related lectins. Crystallization of MIL was also accomplished and the tetragonal crystals diffracted synchrotron X‐rays to a resolution of 2.8 Å.