Open AccessInfluence of temperature during crystallization setup on precipitate formation and crystal shape of a metalloendopeptidase
Author(s) -
Bogdanović Xenia,
Hinrichs Winfried
Publication year - 2011
Publication title -
acta crystallographica section f
Language(s) - English
Resource type - Journals
ISSN - 1744-3091
DOI - 10.1107/s1744309111001783
Subject(s) - crystallization , protein crystallization , precipitation , materials science , chemical engineering , crystal (programming language) , crystallography , chemistry , chromatography , physics , meteorology , computer science , engineering , programming language
It is well known that protein crystallization is affected by several different parameters such as the composition of the reservoir solution, the protein concentration, the pH and the temperature. An effect of different temperatures during setup of crystallization experiments was observed for a metalloendopeptidase (AsaP1 E294A ). Spontaneous protein precipitation was reduced and the crystal shape could be improved by decreasing the temperature during crystallization setup.