Open AccessCrystallization and preliminary X‐ray crystallographic study of a methyltransferase involved in 2‐methylisoborneol biosynthesis in Streptomyces lasaliensis
Author(s) -
Ariyawutthiphan Orapin,
Ose Toyoyuki,
Tsuda Muneya,
Gao YongGui,
Yao Min,
Minami Atsushi,
Oikawa Hideaki,
Tanaka Isao
Publication year - 2011
Publication title -
acta crystallographica section f
Language(s) - English
Resource type - Journals
ISSN - 1744-3091
DOI - 10.1107/s1744309110051523
Subject(s) - biosynthesis , pyrophosphate , stereochemistry , farnesyl pyrophosphate , chemistry , o methyltransferase , crystallization , methyltransferase , cofactor , terpenoid , enzyme , biochemistry , methylation , organic chemistry , dna
The biosynthetic pathway of the off‐flavour terpenoid alcohol 2‐methylisoborneol (2‐MIB) requires geranyl pyrophosphate methyltransferase (GPPMT) to methylate GPP before the cyclization reaction. GPPMT is the first example of an S ‐adenosyl‐ l ‐methionine‐dependent methyltransferase that acts on general intermediates such as geranyl pyrophosphate and farnesyl pyrophosphate in isoprenoid biosynthetic pathways. In this study, recombinant GPPMT was overproduced, purified and crystallized in the absence and presence of cofactor, cofactor analogue and substrate. Well diffracting crystals of apo GPPMT containing one molecule in the asymmetric unit were obtained and the structure of this form was solved by the molecular‐replacement method. Two crystal forms of the tertiary complex with GPP and sinefungin were also obtained. Structure analysis of these crystals is currently under way in order to understand the enzyme reaction mechanism.