Open AccessPreparation, crystallization and preliminary X‐ray diffraction analysis of two intestinal fatty‐acid binding proteins in the presence of 11‐(dansylamino)undecanoic acid
Author(s) -
Laguerre Aisha,
Wielens Jerome,
Parker Michael W.,
Porter Christopher J. H.,
Scanlon Martin J.
Publication year - 2011
Publication title -
acta crystallographica section f
Language(s) - English
Resource type - Journals
ISSN - 1744-3091
DOI - 10.1107/s1744309110051481
Subject(s) - fatty acid binding protein , fatty acid , crystallization , intracellular , biochemistry , molecule , chemistry , peptide sequence , gene , organic chemistry
Fatty‐acid binding proteins (FABPs) are abundantly expressed proteins that bind a range of lipophilic molecules. They have been implicated in the import and intracellular distribution of their ligands and have been linked with metabolic and inflammatory responses in the cells in which they are expressed. Despite their high sequence identity, human intestinal FABP (hIFABP) and rat intestinal FABP (rIFABP) bind some ligands with different affinities. In order to address the structural basis of this differential binding, diffraction‐quality crystals have been obtained of hIFABP and rIFABP in complex with the fluorescent fatty‐acid analogue 11‐(dansylamino)undecanoic acid.