Open AccessPurification, crystallization and preliminary X‐ray analysis of a thermostable direct haemolysin from Grimontia hollisae
Author(s) -
Wang YuKuo,
Huang ShengCih,
Wu YiFang,
Chen YuChing,
Chen WenHung,
Lin YanLing,
Nayak Manoswini,
Lin Yan Ren,
Li Thomas TienHsiung,
Wu TungKung
Publication year - 2011
Publication title -
acta crystallographica section f
Language(s) - English
Resource type - Journals
ISSN - 1744-3091
DOI - 10.1107/s1744309110050219
Subject(s) - orthorhombic crystal system , hemolysin , crystallization , halophile , crystallography , vibrio , solvent , molecule , toxin , cytotoxicity , crystal structure , materials science , chemistry , bacteria , biology , biochemistry , organic chemistry , genetics , virulence , in vitro , gene
Vibrio hollisae , a halophilic species recently reclassified as Grimontia hollisae , is a causative agent of gastroenteritis and septicaemia. One important pathogenic Vibrio factor, thermostable direct haemolysin (TDH), has been purified and crystallized in two crystal forms using the vapour‐diffusion method. The crystals belonged to an orthorhombic space group, with unit‐cell parameters a = 104.8, b = 112.4, c = 61.3 Å and a = 122.9, b = 123.3, c = 89.8 Å. The crystals contained either four or eight molecules per asymmetric unit, with predicted solvent contents of 49.4 and 46.3% and Matthews coefficients ( V M ) of 2.4 and 2.3 Å 3 Da −1 , respectively. These crystals were suitable for structure determination, which would yield structural details related to the cytotoxicity and oligomeric structure of this pore‐forming toxin.