Open AccessCrystallization and preliminary X‐ray analysis of a cold‐active endo‐β‐1,4‐ d ‐xylanase from glycoside hydrolase family 8
Author(s) -
Schoepe Jan,
Pollet Annick,
Verjans Priscilla,
Delcour Jan A.,
Strelkov Sergei V.,
Courtin Christophe M.
Publication year - 2011
Publication title -
acta crystallographica section f
Language(s) - English
Resource type - Journals
ISSN - 1744-3091
DOI - 10.1107/s1744309110048451
Subject(s) - xylanase , crystallization , glycoside hydrolase , resolution (logic) , crystallography , chemistry , solvent , molecule , hydrolase , hydrolysis , stereochemistry , enzyme , biochemistry , organic chemistry , artificial intelligence , computer science
Endo‐β‐1,4‐ d ‐xylanases are used in a multitude of industrial applications. Native crystals of a cold‐adapted xylanase from glycoside hydrolase family 8 were obtained by the vapour‐diffusion technique. The crystals belonged to space group I 222, with unit‐cell parameters a = 46.6, b = 110.8, c = 150.2 Å at 100 K, and diffracted to 2.7 Å resolution at a synchrotron source. The asymmetric unit is likely to contain one molecule, with a V M of 2.07 Å 3 Da −1 , corresponding to a solvent content of ∼40%.