Crystallization and preliminary X‐ray analysis of a cold‐active endo‐β‐1,4‐ d ‐xylanase from glycoside hydrolase family 8

Endo‐β‐1,4‐ d ‐xylanases are used in a multitude of industrial applications. Native crystals of a cold‐adapted xylanase from glycoside hydrolase family 8 were obtained by the vapour‐diffusion technique. The crystals belonged to space group I 222, with unit‐cell parameters a = 46.6, b = 110.8, c = 150.2 Å at 100 K, and diffracted to 2.7 Å resolution at a synchrotron source. The asymmetric unit is likely to contain one molecule, with a V M of 2.07 Å 3  Da −1 , corresponding to a solvent content of ∼40%.