Open AccessPreliminary X‐ray crystallographic analysis of the glycosyltransferase from a marine Streptomyces species
Author(s) -
Gong Liping,
Xiao Yi,
Liu Qiang,
Li Sumei,
Zhang Changsheng,
Liu Jinsong
Publication year - 2011
Publication title -
acta crystallographica section f
Language(s) - English
Resource type - Journals
ISSN - 1744-3091
DOI - 10.1107/s1744309110048268
Subject(s) - glycosyltransferase , crystallization , crystallography , streptomyces , molecule , cloning (programming) , stereochemistry , biosynthesis , chemistry , biology , biochemistry , gene , genetics , organic chemistry , bacteria , computer science , programming language
ElaGT is a glycosyltransferase from a marine Streptomyces species that is involved in the biosynthesis of elaiophylin. Here, the molecular cloning, protein expression and purification, preliminary crystallization and crystallographic characterization of ElaGT are reported. The rod‐shaped crystals belonged to space group P 2 1 22, with unit‐cell parameters a = 66.7, b = 131.7, c = 224.6 Å, α = 90, β = 90, γ = 90°. Data were collected to 2.9 Å resolution. A preliminary molecular‐replacement solution implied the presence of two ElaGT molecules in the asymmetric unit.