Open AccessExpression, purification, crystallization and preliminary crystallographic studies of cis ‐biphenyl‐2,3‐dihydrodiol‐2,3‐dehydrogenase from Pandoraea pnomenusa B‐356
Author(s) -
Patil Dipak N.,
Tomar Shailly,
Sylvestre Michel,
Kumar Pravindra
Publication year - 2010
Publication title -
acta crystallographica section f
Language(s) - English
Resource type - Journals
ISSN - 1744-3091
DOI - 10.1107/s1744309110036894
Subject(s) - biphenyl , crystallization , sodium formate , escherichia coli , solvent , chemistry , dehydrogenase , crystallography , polyethylene glycol , strain (injury) , stereochemistry , enzyme , organic chemistry , biochemistry , biology , anatomy , gene
cis ‐Biphenyl‐2,3‐dihydrodiol‐2,3‐dehydrogenase (BphB) is involved in the aerobic biodegradation of biphenyl and polychlorinated biphenyls. BphB from Pandoraea pnomenusa strain B‐356 was overexpressed in Escherichia coli , purified to homogeneity and crystallized. Crystals were obtained by the sitting‐drop vapour‐diffusion method using polyethylene glycol 3350 and 0.2 M sodium malonate. A BphB crystal diffracted to 2.8 Å resolution and belonged to space group P 4 3 2 1 2, with unit‐cell parameters a = b = 75.2, c = 180.4 Å. Preliminary crystallographic analysis indicated the presence of two molecules in the asymmetric unit, giving a Matthews coefficient of 2.2 Å 3 Da −1 and a solvent content of 44%.