Open AccessCrystallization and preliminary X‐ray studies of native and mutant intimin from enterohaemorrhagic Escherichia coli
Author(s) -
Yi Yong,
Gao Feng,
Mao Xuhu,
Xiao Ming,
Luo Ping,
Qi Jianxun,
Guo Gang,
Jing Hua,
Cui Yan,
Zou Quanming
Publication year - 2011
Publication title -
acta crystallographica section f
Language(s) - English
Resource type - Journals
ISSN - 1744-3091
DOI - 10.1107/s1744309110036432
Subject(s) - intimin , orthorhombic crystal system , escherichia coli , bacterial outer membrane , crystallization , mutant , microbiology and biotechnology , bacteria , biology , crystallography , chemistry , crystal structure , enterobacteriaceae , biochemistry , gene , organic chemistry , genetics
Enterohaemorrhagic Escherichia coli (EHEC) O157:H7 is a primarily food‐borne bacterial pathogen that is capable of causing life‐threatening human infections and poses a serious challenge to public health worldwide. The bacterial outer‐membrane protein intimin plays a key role in the initiation process of EHEC infection. In this study, intimin from EHEC O157:H7 (Int188) and its N916Y mutant (IntN916Y) were purified and crystals of both were obtained using the hanging‐drop vapour‐diffusion method at 291 K. Data were collected from Int188 and IntN916Y crystals to 2.8 and 2.6 Å resolution, respectively. The crystal of Int188 belonged to the orthorhombic space group C 2, with unit‐cell parameters a = 235.16, b = 44.81, c = 129.12 Å, α = γ = 90, β = 97.53°. The crystal of IntN916Y belonged to space group P 2 1 2 1 2 1 , with unit‐cell parameters a = 43.78, b = 92.49, c = 100.05 Å, α = β = γ = 90°.