Crystallization and preliminary X‐ray diffraction studies of a terminal oxygenase of carbazole 1,9a‐dioxygenase from Novosphingobium sp. KA1

Carbazole 1,9a‐dioxygenase (CARDO) is the initial dioxygenase in the carbazole‐degradation pathway of Novosphingobium sp. KA1. The CARDO from KA1 consists of a terminal oxygenase (Oxy), a putidaredoxin‐type ferredoxin and a ferredoxin reductase. The Oxy from Novosphingobium sp. KA1 was crystallized at 277 K using the hanging‐drop vapour‐diffusion method with ammonium sulfate as the precipitant. Diffraction data were collected to a resolution of 2.1 Å. The crystals belonged to the monoclinic space group P 2 1 . Self‐rotation function analysis suggested that the asymmetric unit contained two Oxy trimers; the Matthews coefficient and solvent content were calculated to be 5.9 Å 3  Da −1 and 79.1%, respectively.