Crystallization and preliminary X‐ray diffraction analysis of red clover necrotic mosaic virus | Zendy

Martin Stanton L. | Zendy; Guenther Richard H. | Zendy; Sit Tim L. | Zendy; Swartz Paul D. | Zendy; Meilleur Flora | Zendy; Lommel Steven A. | Zendy; Rose Robert B. | Zendy

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Crystallization and preliminary X‐ray diffraction analysis of red clover necrotic mosaic virus

Author(s) -

Martin Stanton L.,

Guenther Richard H.,

Sit Tim L.,

Swartz Paul D.,

Meilleur Flora,

Lommel Steven A.,

Rose Robert B.

Publication year - 2010

Publication title -

acta crystallographica section f

Language(s) - English

Resource type - Journals

ISSN - 1744-3091

DOI - 10.1107/s1744309110032483

Subject(s) - icosahedral symmetry , x ray , capsid , crystallography , resolution (logic) , virology , x ray crystallography , diffraction , biology , crystallization , virus , materials science , chemistry , optics , physics , organic chemistry , artificial intelligence , computer science

Red clover necrotic mosaic virus (RCNMV) is a species that belongs to the Tombusviridae family of plant viruses with a T = 3 icosahedral capsid. RCNMV virions were purified and were crystallized for X‐ray analysis using the hanging‐drop vapor‐diffusion method. Self‐rotation functions and systematic absences identified the space group as I 23, with two virions in the unit cell. The crystals diffracted to better than 4 Å resolution but were very radiation‐sensitive, causing rapid decay of the high‐resolution reflections. The data were processed to 6 Å in the analysis presented here.

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