Open AccessCrystallization and preliminary X‐ray diffraction analysis of red clover necrotic mosaic virus
Author(s) -
Martin Stanton L.,
Guenther Richard H.,
Sit Tim L.,
Swartz Paul D.,
Meilleur Flora,
Lommel Steven A.,
Rose Robert B.
Publication year - 2010
Publication title -
acta crystallographica section f
Language(s) - English
Resource type - Journals
ISSN - 1744-3091
DOI - 10.1107/s1744309110032483
Subject(s) - icosahedral symmetry , x ray , capsid , crystallography , resolution (logic) , virology , x ray crystallography , diffraction , biology , crystallization , virus , materials science , chemistry , optics , physics , organic chemistry , artificial intelligence , computer science
Red clover necrotic mosaic virus (RCNMV) is a species that belongs to the Tombusviridae family of plant viruses with a T = 3 icosahedral capsid. RCNMV virions were purified and were crystallized for X‐ray analysis using the hanging‐drop vapor‐diffusion method. Self‐rotation functions and systematic absences identified the space group as I 23, with two virions in the unit cell. The crystals diffracted to better than 4 Å resolution but were very radiation‐sensitive, causing rapid decay of the high‐resolution reflections. The data were processed to 6 Å in the analysis presented here.