Open AccessCrystallization and preliminary X‐ray analysis of a marine diatom‐infecting single‐stranded RNA virus
Author(s) -
Naitow Hisashi,
Shirai Yoko,
Tomaru Yuji,
Nagasaki Keizo
Publication year - 2010
Publication title -
acta crystallographica section f
Language(s) - English
Resource type - Journals
ISSN - 1744-3091
DOI - 10.1107/s1744309110032422
Subject(s) - crystallization , diatom , virus , rna , x ray , crystallography , virology , biology , materials science , chemistry , physics , optics , genetics , gene , ecology , organic chemistry
Crystals of a diatom‐infecting virus (CtenRNAV) that diffracted to a resolution of 4.0 Å were grown in a mixture of 2‐methyl‐2,4‐pentanediol (MPD), calcium chloride and sodium acetate. It was possible to freeze the crystals directly at liquid‐nitrogen temperature as the reservoir solution, which included about 30% MPD, acted as a cryoprotectant during X‐ray diffraction data collection. A data set was collected from a single frozen crystal obtained using this method. The crystals belonged to space group P 6 3 22, with unit‐cell parameters a = b = 448.67, c = 309.76 Å and two virus particles in the unit cell. The virus‐particle orientation was determined using a rotation function and the virus‐particle centre was estimated on the basis of crystallographic considerations. The packing of CtenRNAV in the crystal lattice was revealed by this preliminary crystallographic study.