Open AccessExpression, purification and crystallization of Chaetomium thermophilum Cu,Zn superoxide dismutase
Author(s) -
Wakadkar Sachin,
Zhang LiQing,
Li DuoChuan,
Haikarainen Teemu,
Dhavala Prathusha,
Papageorgiou Anastassios C.
Publication year - 2010
Publication title -
acta crystallographica section f
Language(s) - English
Resource type - Journals
ISSN - 1744-3091
DOI - 10.1107/s1744309110030393
Subject(s) - superoxide dismutase , crystallization , chemistry , chaetomium , biochemistry , enzyme , food science , organic chemistry , penicillium
Cu,Zn superoxide dismutase (Cu,ZnSOD) from the thermophilic fungus Chaetomium thermophilum was expressed in Pichia pastoris and purified. Crystals were grown in over 120 conditions but only those produced with 1.4 M sodium potassium phosphate pH 8.2 as precipitant were suitable for structural studies. Data were collected to 1.9 Å resolution at 100 K from a single crystal using a synchrotron‐radiation source. The crystals belonged to space group P 6 1 / P 6 5 , with unit‐cell parameters a = 90.2, c = 314.5 Å and eight molecules in the asymmetric unit. Elucidation of the crystal structure will provide insights into the active site of the enzyme and a better understanding of the structure–activity relationship, assembly and thermal stability of Cu,ZnSODs.