Open AccessCrystallization and preliminary X‐ray diffraction analysis of the Thermoanaerobacter ethanolicus secondary alcohol dehydrogenase I86A mutant
Author(s) -
Protsko Carla,
Vieille Claire,
Laivenieks Maris,
Prasad Lata,
Sanders David A. R.,
Delbaere Louis T. J.
Publication year - 2010
Publication title -
acta crystallographica section f
Language(s) - English
Resource type - Journals
ISSN - 1744-3091
DOI - 10.1107/s1744309110018981
Subject(s) - orthorhombic crystal system , alcohol dehydrogenase , crystallography , stereospecificity , stereochemistry , crystallization , alcohol oxidoreductase , resolution (logic) , chemistry , mutant , crystal structure , alcohol , enzyme , nad+ kinase , biochemistry , organic chemistry , catalysis , artificial intelligence , computer science , gene
The Thermoanaerobacter ethanolicus secondary alcohol dehydrogenase I86A mutant is stereospecific for ( R )‐alcohols instead of ( S )‐alcohols. Pyramidal crystals grown in the presence of ( R )‐phenylethanol via the hanging‐drop vapour‐diffusion method diffracted to 3.2 Å resolution at the Canadian Light Source. The crystal belonged to the orthorhombic space group P 2 1 2 1 2 1 , with unit‐cell parameters a = 80.23, b = 124.90, c = 164.80 Å. The structure was solved by molecular replacement using the structure of T. brockii SADH (PDB entry 1ykf ).