Crystallization and preliminary diffraction analysis of an engineered cephalosporin acylase | Zendy

Anandan Anandhi | Zendy; Vallet Corinne | Zendy; Coyle Travis | Zendy; Moustafa Ibrahim M. | Zendy; Vrielink Alice | Zendy

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Crystallization and preliminary diffraction analysis of an engineered cephalosporin acylase

Author(s) -

Anandan Anandhi,

Vallet Corinne,

Coyle Travis,

Moustafa Ibrahim M.,

Vrielink Alice

Publication year - 2010

Publication title -

acta crystallographica section f

Language(s) - English

Resource type - Journals

ISSN - 1744-3091

DOI - 10.1107/s1744309110017185

Subject(s) - crystallization , materials science , cephalosporin , chemical engineering , chemistry , biochemistry , engineering , antibiotics

Crystallization conditions are reported for an engineered cephalosporin acylase based on the sequence of glutaryl‐7‐aminocephalosporanic acid acylase from Pseudomonas strain N176. Initial crystals were grown using polyethylene glycol as a crystallizing agent; however, these crystals diffracted poorly and exhibited high mosaicity. A dehydration procedure in which crystals were transferred to a solution containing a higher concentration of polyethylene glycol as well as glycerol improved the diffraction quality such that a 1.57 Å diffraction data set could be obtained.

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