Open AccessCrystallization and preliminary crystallographic analysis of the Clostridium perfringens enterotoxin
Author(s) -
Briggs David C.,
Smedley James G.,
McClane Bruce A.,
Basak Ajit K.
Publication year - 2010
Publication title -
acta crystallographica section f
Language(s) - English
Resource type - Journals
ISSN - 1744-3091
DOI - 10.1107/s1744309110016507
Subject(s) - clostridium perfringens , enterotoxin , crystallization , microbiology and biotechnology , pore forming toxin , clostridium , bacteria , toxin , crystal (programming language) , microbial toxins , chemistry , escherichia coli , crystallography , biology , biochemistry , genetics , organic chemistry , gene , computer science , programming language
Clostridium perfringens is a Gram‐positive anaerobic species of bacterium that is notable for its ability to produce a plethora of toxins, including membrane‐active toxins (α‐toxins), pore‐forming toxins (ɛ‐toxins) and binary toxins (ι‐toxins). Here, the crystallization of the full‐length wild‐type C. perfringens enterotoxin is reported, which is the causative agent of the second most prevalent food‐borne illness in the United States and has been implicated in many other gastrointestinal pathologies. Several crystal forms were obtained. However, only two of these optimized crystal forms (I and II) were useable for X‐ray diffraction data collection. The form I crystals diffracted to d min = 2.7 Å and belonged to space group C 2, while the form II crystals diffracted to d min = 4 Å and belonged to space group P 2 1 3.