Open AccessCrystallization and preliminary crystallographic studies of CorC, a magnesium‐ion transporter
Author(s) -
Zhang Ning,
Ren Xiaoming,
Zhu Deyu,
Li Defeng,
Wang Dacheng
Publication year - 2010
Publication title -
acta crystallographica section f
Language(s) - English
Resource type - Journals
ISSN - 1744-3091
DOI - 10.1107/s1744309110012613
Subject(s) - crystallization , magnesium , crystallography , materials science , transporter , ion , chemistry , biochemistry , metallurgy , organic chemistry , gene
CorC is a magnesium transporter that is involved in the Mg 2+ ‐efflux function of the CorA transporter system, an Mg 2+ channel, from Shigella flexneri . Native CorC was purified and crystallized in the native form and in a ligand‐free form and diffraction data sets were collected to 2.9 and 3.4 Å resolution, respectively. The native CorC crystals belonged to space group P 2 1 2 1 2 1 , with unit‐cell parameters a = 64.31, b = 74.44, c = 132.78 Å. The ligand‐free CorC crystals belonged to space group P 3 1 21/ P 3 2 21, with unit‐cell parameters a = b = 71.89, c = 125.96 Å. The CorC–ATP complex has also been crystallized and the crystals belonged to space group P 2 or P 2 1 .