Open AccessCrystallization and preliminary X‐ray crystallographic analysis of MinE, the cell‐division topological specificity factor from Helicobacter pylori
Author(s) -
Kang Gil Bu,
Song HyeEun,
Kim MunKyoung,
Youn HyungSeop,
An Jun Yop,
Lee JungGyu,
Park Kyung Ryung,
Eom Soo Hyun
Publication year - 2010
Publication title -
acta crystallographica section f
Language(s) - English
Resource type - Journals
ISSN - 1744-3091
DOI - 10.1107/s1744309110009784
Subject(s) - ftsz , crystallization , crystallography , resolution (logic) , cell division , division (mathematics) , helicobacter pylori , ring (chemistry) , crystal structure , bacterial cell structure , crystal (programming language) , molecule , chemistry , bacteria , stereochemistry , topology (electrical circuits) , cell , biology , mathematics , genetics , biochemistry , combinatorics , computer science , arithmetic , organic chemistry , artificial intelligence , programming language
Cell division in Gram‐negative bacteria is driven by the formation of an FtsZ ring at the division site. MinE regulates the proper placement of the FtsZ ring at mid‐cell by blocking the inhibitory action of the MinCD complex. Diffraction data were collected at 2.8 Å resolution from a native crystal of full‐length Helicobacter pylori MinE. The crystal belonged to space group P 6 4 . Assuming the presence of two molecules in the asymmetric unit, the calculated Matthews coefficient was 2.58 Å 3 Da −1 , which corresponds to a solvent content of 52.3%. For MAD phasing, a four‐wavelength data set was collected at 3.0 Å resolution.