Open AccessX‐ray investigation of gene‐engineered human insulin crystallized from a solution containing polysialic acid
Author(s) -
Timofeev V. I.,
ChuprovNetochin R. N.,
Samigina V. R.,
Bezuglov V. V.,
Miroshnikov K. A.,
Kuranova I. P.
Publication year - 2010
Publication title -
acta crystallographica section f
Language(s) - English
Resource type - Journals
ISSN - 1744-3091
DOI - 10.1107/s1744309110000461
Subject(s) - polysialic acid , protein data bank (rcsb pdb) , crystallography , human insulin , molecule , recombinant dna , molecular replacement , insulin , crystal structure , crystal (programming language) , chemistry , biochemistry , gene , cell , biology , cell adhesion , organic chemistry , endocrinology , neural cell adhesion molecule , computer science , programming language
Attempts to crystallize the noncovalent complex of recombinant human insulin with polysialic acid were carried out under normal and microgravity conditions. Both crystal types belonged to the same space group, I 2 1 3, with unit‐cell parameters a = b = c = 77.365 Å, α = β = γ = 90.00°. The reported space group and unit‐cell parameters are almost identical to those of cubic insulin reported in the PDB. The results of X‐ray studies confirmed that the crystals obtained were cubic insulin crystals and that they contained no polysialic acid or its fragments. Electron‐density maps were calculated using X‐ray diffraction sets from earth‐grown and microgravity‐grown crystals and the three‐dimensional structure of the insulin molecule was determined and refined. The conformation and secondary‐structural elements of the insulin molecule in different crystal forms were compared.