Crystallization and preliminary X‐ray analysis of the diadenosine 5′,5′′′‐ P 1 , P 4 ‐tetraphosphate phosphorylase from Mycobacterium tuberculosis H37Rv

A novel diadenosine 5′,5′′′‐ P 1 , P 4 ‐tetraphosphate (Ap4A) phosphorylase (Rv2613c) from Mycobacterium tuberculosis H37Rv has been crystallized by the sitting‐drop vapour‐diffusion method. The crystal belonged to space group C 2, with unit‐cell parameters a = 101.5, b = 63.6, c = 79.1 Å, β = 110.9°. The diffraction of the crystals extended to 1.9 Å resolution. The asymmetric unit is expected to contain two molecules of Rv2613c, with a corresponding crystal volume per protein weight ( V M ) of 2.41 Å 3  Da −1 and a solvent content of 49.1%. This is the first report of a crystal of Ap4A phosphorylase.