Open AccessCrystallization and preliminary X‐ray analysis of the MaoC‐like dehydratase from Phytophthora capsici
Author(s) -
Wang Huizheng,
Guo Jiubiao,
Pang Hai,
Zhang Xiuguo
Publication year - 2010
Publication title -
acta crystallographica section f
Language(s) - English
Resource type - Journals
ISSN - 1744-3091
DOI - 10.1107/s1744309109053391
Subject(s) - orthorhombic crystal system , crystallization , dehydratase , x ray , biosynthesis , chemistry , crystallography , materials science , crystal structure , biochemistry , enzyme , organic chemistry , physics , optics
MaoC‐like dehydratase (MaoC) plays an important role in supplying ( R )‐3‐hydroxyacyl‐CoA from the fatty‐acid oxidation pathway to polyhydroxyalkanoate (PHA) biosynthetic pathways. PHAs have been attracting much attention as they can be used in the biosynthesis of synthetic plastics. Crystals of MaoC from Phytophora capsici were grown by the hanging‐drop vapour‐diffusion method at 289 K in a number of screening conditions. An MaoC crystal diffracted to 1.93 Å resolution using X‐ray radiation and belonged to the orthorhombic space group P 2 1 2 1 2 1 , with unit‐cell parameters a = 81.458, b = 82.614, c = 124.228 Å, α = β = γ = 90°.