Open AccessCrystallization and preliminary crystallographic analysis of eukaryotic transcription and mRNA export factor Iws1 from Encephalitozoon cuniculi
Author(s) -
Koch Michael,
Diebold MarieLaure,
Cavarelli Jean,
Romier Christophe
Publication year - 2010
Publication title -
acta crystallographica section f
Language(s) - English
Resource type - Journals
ISSN - 1744-3091
DOI - 10.1107/s1744309109052749
Subject(s) - encephalitozoon cuniculi , messenger rna , crystallization , transcription (linguistics) , eukaryotic transcription , biology , rna polymerase ii , crystallography , elongation , rna , microbiology and biotechnology , chemistry , promoter , gene , biochemistry , materials science , gene expression , microsporidia , linguistics , philosophy , spore , organic chemistry , ultimate tensile strength , metallurgy
Transcription elongation by eukaryotic RNA polymerase II requires the coupling of mRNA synthesis and mRNA processing and export. The essential protein Iws1 is at the interface of these processes through its interaction with histone chaperone and elongation factor Spt6 as well as with complexes involved in mRNA processing and export. Upon crystallization of the evolutionarily conserved domain of Iws1 from Encephalitozoon cuniculi , four different crystal forms were obtained. Three of the crystal forms belonged to space group P 2 1 and one belonged to space group P 222 1 . Preliminary X‐ray crystallographic analysis of one of the crystal forms allowed the collection of data to 2.5 Å resolution.