Open AccessPurification, crystallization and preliminary crystallographic analysis of the minor pilin FctB from Streptococcus pyogenes
Author(s) -
Linke Christian,
Young Paul G.,
Kang Hae Joo,
Proft Thomas,
Baker Edward N.
Publication year - 2010
Publication title -
acta crystallographica section f
Language(s) - English
Resource type - Journals
ISSN - 1744-3091
DOI - 10.1107/s1744309109051951
Subject(s) - pilin , pilus , streptococcus pyogenes , crystallization , crystallography , microbiology and biotechnology , materials science , chemistry , escherichia coli , biology , gene , bacteria , biochemistry , genetics , organic chemistry , staphylococcus aureus
The minor pilin FctB is an integral part of the pilus assembly expressed by Streptococcus pyogenes . Since it is located at the cell wall, it can be hypothesized that it functions as a cell‐wall anchor for the streptococcal pilus. In order to elucidate its structure, the genes for FctB from the S. pyogenes strains 90/306S and SF370 were cloned for overexpression in Escherichia coli . FctB from strain 90/306S was crystallized by the sitting‐drop vapour‐diffusion method using sodium citrate as a precipitant. The hexagonal FctB crystals belonged to space group P 6 1 or P 6 5 , with unit‐cell parameters a = b = 95.15, c = 100.25 Å, and diffracted to 2.9 Å resolution.