Open AccessStructure of bovine pancreatic ribonuclease complexed with uridine 5′‐monophosphate at 1.60 Å resolution
Author(s) -
Larson Steven B.,
Day John S.,
Nguyen Chieugiang,
Cudney Robert,
McPherson Alexander
Publication year - 2010
Publication title -
acta crystallographica section f
Language(s) - English
Resource type - Journals
ISSN - 1744-3091
DOI - 10.1107/s174430910905194x
Subject(s) - bovine pancreatic ribonuclease , rnase p , ribonuclease , pyrimidine , chemistry , uridine , pancreatic ribonuclease , enzyme , ribonuclease iii , stereochemistry , biochemistry , rna , rna interference , gene
Bovine pancreatic ribonuclease A (RNase A) was crystallized from a mixture of small molecules containing basic fuchsin, tobramycin and uridine 5′‐monophosphate (U5P). Solution of the crystal structure revealed that the enzyme was selectively bound to U5P, with the pyrimidine ring of U5P residing in the pyrimidine‐binding site at Thr45. The structure was refined to an R factor of 0.197 and an R free of 0.253.