Open AccessTowards structural studies of the old yellow enzyme homologue SYE4 from Shewanella oneidensis and its complexes at atomic resolution
Author(s) -
Elegheert Jonathan,
Van Den Hemel Debbie,
Dix Ina,
Stout Jan,
Van Beeumen Jozef,
Brigé Ann,
Savvides Savvas N.
Publication year - 2010
Publication title -
acta crystallographica section f
Language(s) - English
Resource type - Journals
ISSN - 1744-3091
DOI - 10.1107/s1744309109050386
Subject(s) - shewanella oneidensis , enzyme , escherichia coli , crystallography , resolution (logic) , chemistry , bacteria , biology , biochemistry , gene , genetics , artificial intelligence , computer science
Shewanella oneidensis is an environmentally versatile Gram‐negative γ‐proteobacterium that is endowed with an unusually large proteome of redox proteins. Of the four old yellow enzyme (OYE) homologues found in S. oneidensis , SYE4 is the homologue most implicated in resistance to oxidative stress. SYE4 was recombinantly expressed in Escherichia coli , purified and crystallized using the hanging‐drop vapour‐diffusion method. The crystals belonged to the orthorhombic space group P 2 1 2 1 2 1 and were moderately pseudo‐merohedrally twinned, emulating a P 422 metric symmetry. The native crystals of SYE4 were of exceptional diffraction quality and provided complete data to 1.10 Å resolution using synchrotron radiation, while crystals of the reduced enzyme and of the enzyme in complex with a wide range of ligands typically led to high‐quality complete data sets to 1.30–1.60 Å resolution, thus providing a rare opportunity to dissect the structure–function relationships of a good‐sized enzyme (40 kDa) at true atomic resolution. Here, the attainment of a number of experimental milestones in the crystallographic studies of SYE4 and its complexes are reported, including isolation of the elusive hydride–Meisenheimer complex.