Open AccessA preliminary X‐ray study of d , d ‐heptose‐1,7‐bisphosphate phosphatase from Burkholderia thailandensis E264
Author(s) -
Kim MiSun,
Shin Dong Hae
Publication year - 2010
Publication title -
acta crystallographica section f
Language(s) - English
Resource type - Journals
ISSN - 1744-3091
DOI - 10.1107/s1744309109042614
Subject(s) - heptose , burkholderia pseudomallei , burkholderia , microbiology and biotechnology , biology , biosynthesis , bacteria , biochemistry , enzyme , genetics , mutant , gene
d , d ‐Heptose‐1,7‐bisphosphate phosphatase (GmhB), which is involved in the third step of the NDP‐heptose biosynthesis pathway, converts d , d ‐heptose‐1,7‐bisphosphate to d , d ‐heptose‐1‐phosphate. This biosynthesis pathway is a target for new antibiotics or antibiotic adjuvants for Gram‐negative pathogens. Burkholderia thailandensis is a useful surrogate organism for studying the pathogenicity of melioidosis owing to its extensive genomic similarity to B. pseudomallei . Melioidosis caused by B. pseudomallei is a serious invasive disease of animals and humans in tropical and subtropical areas. In this study, GmhB has been cloned, expressed, purified and crystallized. X‐ray data have also been collected to 2.50 Å resolution using synchrotron radiation. The crystal belonged to space group P 6, with unit‐cell parameters a = 243.2, b = 243.2, c = 41.1 Å.