Open AccessCrystallization of the head and galectin‐like domains of porcine adenovirus isolate NADC‐1 fibre
Author(s) -
GuardadoCalvo Pablo,
LlamasSaiz Antonio L.,
Fox Gavin C.,
Glasgow Joel N.,
Van Raaij Mark J.
Publication year - 2009
Publication title -
acta crystallographica section f
Language(s) - English
Resource type - Journals
ISSN - 1744-3091
DOI - 10.1107/s1744309109038287
Subject(s) - crystallization , domain (mathematical analysis) , angstrom , head (geology) , crystallography , biophysics , c terminus , resolution (logic) , galectin , chemistry , biology , microbiology and biotechnology , biochemistry , computer science , amino acid , mathematical analysis , paleontology , mathematics , organic chemistry , artificial intelligence
The porcine adenovirus NADC‐1 isolate, a strain of porcine adenovirus type 4, has a fibre with an atypical architecture. In addition to a classical virus‐attachment region, shaft and head domains, it contains an additional galectin‐like domain C‐terminal to the head domain and connected to the head domain by a long RGD‐containing loop. The galectin‐like domain contains two putative carbohydrate‐recognition domains. The head and galectin‐like domains have been independently crystallized. Diffraction data have been obtained to 3.2 Å resolution from crystals of the head domain and to 1.9 Å resolution from galectin‐like domain crystals.