Open AccessHow to get the magic triangle and the MAD triangle into your protein crystal
Author(s) -
Beck Tobias,
Da Cunha Carlos Eduardo,
Sheldrick George M.
Publication year - 2009
Publication title -
acta crystallographica section f
Language(s) - English
Resource type - Journals
ISSN - 1744-3091
DOI - 10.1107/s1744309109036884
Subject(s) - phaser , magic (telescope) , crystallography , protein crystallization , amino acid , amino acid residue , trypsin , chemistry , combinatorics , stereochemistry , physics , mathematics , peptide sequence , biochemistry , crystallization , organic chemistry , enzyme , optics , quantum mechanics , gene
The magic triangle 5‐amino‐2,4,6‐triiodoisophthalic acid (I3C) and the MAD triangle 5‐amino‐2,4,6‐tribromoisophthalic acid (B3C) are two representatives of a novel class of compounds that combine heavy atoms for experimental phasing with functional groups for protein interactions. These compounds are readily available and provide easy access to experimental phasing. The preparation of stock solutions and the incorporation of the compounds into protein crystals are discussed. As an example of incorporation via cocrystallization, the incorporation of B3C into bovine trypsin, resulting in a single site with high occupancy, is described.