Open AccessCrystallization and preliminary X‐ray diffraction characterization of RpfF, a key DSF synthase from Stenotrophomonas maltophilia
Author(s) -
Lin XianYa,
Liao ShuJu,
Chin KoHsin,
Shih HuiLing,
Shen GwanHan,
Wang Andrew H.J.,
Chou ShanHo
Publication year - 2009
Publication title -
acta crystallographica section f
Language(s) - English
Resource type - Journals
ISSN - 1744-3091
DOI - 10.1107/s1744309109033156
Subject(s) - stenotrophomonas maltophilia , atp synthase , stenotrophomonas , crystallization , characterization (materials science) , microbiology and biotechnology , chemistry , materials science , biochemistry , bacteria , enzyme , biology , nanotechnology , organic chemistry , pseudomonas aeruginosa , genetics , gene , 16s ribosomal rna
Stenotrophomonas maltophilia has emerged as a critical nosocomial opportunistic pathogen in the last few years. It is resistant to many clinically useful antibiotics; hence, new ways of combatting this bacterium are essential. Diffusible signal factor (DSF) dependent quorum sensing is a major mechanism of virulence induction in S. maltophilia , with RpfF playing a key role in DSF biosynthesis. Inhibiting S. maltophilia RpfF ( Sm RpfF) function via small‐molecule interference may constitute a new way of treating S. maltophilia infection. Sm RpfF was therefore overexpressed in Escherichia coli , purified and crystallized using the hanging‐drop vapour‐diffusion method. The crystals belonged to the tetragonal space group P 4 1 2 1 2 or P 4 3 2 1 2, with unit‐cell parameters a = b = 148.51, c = 122.82 Å, and diffracted to a resolution of 2.25 Å.