Open AccessStructures of two truncated phage‐tail hyaluronate lyases from Streptococcus pyogenes serotype M1
Author(s) -
MartinezFleites Carlos,
Smith Nicola L.,
Turkenburg Johan P.,
Black Gary W.,
Taylor Edward J.
Publication year - 2009
Publication title -
acta crystallographica section f
Language(s) - English
Resource type - Journals
ISSN - 1744-3091
DOI - 10.1107/s1744309109032813
Subject(s) - streptococcus pyogenes , serotype , microbiology and biotechnology , biology , virology , bacteria , genetics , staphylococcus aureus
The crystal structures of truncated forms of the Streptococcus pyogenes phage‐encoded hyaluronate lyases HylP2 and HylP3 were determined by molecular replacement to 1.6 and 1.9 Å resolution, respectively. The truncated forms crystallized in a hexagonal space group, forming a trimer around the threefold crystallographic axis. The arrangement of the fold is very similar to that observed in the structure of the related hyaluronate lyase HylP1. The structural elements putatively involved in substrate recognition are found to be conserved in both the HylP2 and HylP3 fragments.